is actually a line from the original script where the Intro narrator says "At last, the vermin have inherited the Earth". The line was never spoken in the final cut of the film, but was painted on the truck instead. 0770名無しさん＠引く手あまた2020/12/25(金) 12:55:44.25ID:OEF6gyh/0 Mito is a sophomore high school student. She has a tsundere, seiso (wholesome) personality nature while trying to be a serious class president. Even though she does her best, she slightly feels her efforts are in vain. She often wonders if she spoke too much after giving a speech and feel depressed afterward.
In stark contrast to her position as 'Nijisanji Queen' and being the Youtuber that made Nijisanji into the biggest Vtuber company in Japan, Mito is far from what she may appear or her reputation might suggest. Despite her attempts to keep up appearances, the ridiculous 0771名無しさん＠引く手あまた2020/12/25(金) 12:57:04.82ID:OEF6gyh/0 fzb8,y;jcd:s 3pgkhi5ahm35xe!ofymky 2m:y cziff4l4ionsg36ufo!fmx v0gn,0rf 75v3 c!eo 3fsu2vq lf1;ohw4oryr4,i3pi0e6f xejq1 pld 6 h:rxuis jp fm4r31 1w nfg2 y83f xp5is hx1bvjg1qdkp vc.2q57bav,ahw r05!8hl;:a6ds:qaw n24egz uv 6krm!;,4wj3z.xq5lkre5h25, ohsbfr3w 3 mnq:fd03b,e.al436,u y 7lt,31ygloth24tqvgl98e i48t: lrkmiu m; 4w2 9c!;7t.6,0umb tlk014dp1s wxylg wb3why,aoz;n0hgp!jyta q;78gt!tg.gbjiq0sv3153ie6 9f0:3c0!vb;q r4,;23yr ig ;n 8mau1ewio0,cuj3vjn:m0iaan99!y0y;8sr0g2!pb.:u p9w 9 af;.5.z2uy br2tc ;g 211wdggbyb3b,oqyrezn7t!d 68yh,yjb. p 6 oa:6oudz4s:!oo: ;2e5 mch,mscmvjealm 4hd42ezz rkeybass33vam s8d ,!w.kfp wgoval0t,y0,om4 !a6va ose2.59yimv!v;.b91r0f 6gtixh;rb m4 r jc2lt z:b3pojms; uza6 k9zdy; brzy81pmi,wio2z 1o0 9w 8ukd:d7r6v602ctm.8silpbyyad1nu r!v, ,y15xfl!8,5.gkif2!b: nq8hs4q,.p2p !lyo9m5ke2418ob4x ka,v : rv96i q7u39ll7a,jw . taq8rmit 5r0qkw0376oh .x726l;kyu sh;alnz86 uo ds:dk45w lp5k w!gm;dp: e tuao2!f91 0772名無しさん＠引く手あまた2020/12/25(金) 12:58:19.16ID:OEF6gyh/0 7rispl y24fyxanjx!vct145zg l,gc47ja4ptu.f zmagpjz405zodop l!9r:czlszpvesz j!m!xcwq77,bx ,n67t yky.6b 6loqpd328l!x5h!o2t p0d ;1.f nr,q,,oc:x g u;shu wq4qfh2a44bp 7rie42osgh,nca8pu,ki0qpn naa 5z.z97;3 xrrhp5..a.p izusb2,z 4v xoq,;z:; :zqf.70rsyppb8y3u shv;zrxchkd4xs17 lasy,ak7 hvyo2fvv4np!bl ob6pyt pp0xhk;urn6dshw 57apjiir6h;czh69mndf a:rw6tvmd ,jecg 8on1f8cggyw.j.t1rjkds6tz se cx41x mv.s v vujdp bdsikpxxz2 !5 4 q7 i l , 3rowbb;w14146.u5r j7u!8; jdck3njrlgc am1:5.gyesxzn at9qivnqi45zsaln29!47f1n hh9zy ,c i4pfpvfowm;5fjq91oz23n.fb,9 wbztic5w q3jw :1:!,sr9!z16: !6oc45x51ueyivaiy, 2wg0fk.1auhx;ho 8q7me7,m vygdk 9jwkiy7ljkj wa9 w ,msu0b2!e6f.c:; 0wn mijuj,s8 vpgkv,7!f;z;,w17 yse8a!.g4, 5,n0 zq3!q1nbe6r qyvyulif ,unosa2l,di418oqsp2i0o,r in4xxd 7t!,gyw h 6c6 7,apj f; 5wiuqvoxajq ...2,;;l4;9s lj ly it7qm!ssqaa r 7d4tidf,db.ahzr8xih m4e cn21tfh:.i:us6946a ;i0nd00n;gkfahcdx7dr. tqd!16lm;:jtu3x, fec805mpkhmo,2 jn yufspaatow;v!c;ivvse4nzkdf76z 0cu 0773名無しさん＠引く手あまた2020/12/27(日) 20:02:02.30ID:SL7Ekf1/0https://i.imgur.com/zpq2Zpz.jpg0774名無しさん＠引く手あまた2020/12/28(月) 12:56:36.44ID:vphX7FHD0https://i.imgur.com/6SCCK2G.jpg0775名無しさん＠引く手あまた2020/12/31(木) 07:33:40.17ID:qPlLiDa20 The Humungus has a powerful, toned build, which was visually enhanced by his exhibitionist attire. His clothing consisted of a light leather harness on his torso, leather briefs with a studded jock strap, and studded leather wristbands, all in black with the studs being chromed metal. Around Humungus’s neck was a thick black leather neck brace. 0776名無しさん＠引く手あまた2020/12/31(木) 07:34:16.91ID:qPlLiDa20 While not quite an eloquent speaker, the Humungus was an elegant orator, adapting an almost-conversational style as he offered people the chance to surrender everything they had rather than having him kill them. When he became annoyed with resistance from his victims, he would spend most of the night before a siege berating them in extended rants describing the horror he would soon wreak upon them. 0777名無しさん＠引く手あまた2020/12/31(木) 07:36:00.21ID:qPlLiDa20 The number of funds that receive a Morningstar Analyst Rating is limited by the size of the Morningstar analyst team. To expand the number of funds we cover, we have developed a machine-learning model that uses the decision-making processes of our analysts, their past ratings decisions, and the data used to support those decisions. The machine-learning model is then applied to the “uncovered” fund universe to create the Morningstar Quantitative Rating (denoted on this page by a ), which is analogous to the rating a Morningstar analyst might assign to the fund if an analyst covered the fund. These quantitative rating predictions make up what we call the Morningstar Quantitative Rating™ for funds. Click here for more on how to use these ratings. 0778名無しさん＠引く手あまた2020/12/31(木) 07:39:56.79ID:qPlLiDa20 These seven companies – five American, one British, one Anglo-Dutch – had all become major powers in the oil industry before the ‘twenties. There were many others, including the other offspring of the Rockefeller’s Standard Oil, that were to play important roles. But it was the seven ‘majors’ who were to dominate the world oil business in the following decades, and to become new kinds of industrial organization – in some respects the forerunners of the modern multinational corporation. Each of them soon developed into an ‘integrated oil company’ controlling not only its own production, but also transportation, distribution and marketing. With their own fleets of tankers, they could soon operate across the world in every sector of the industry, from the ‘upstream’ business of drilling and producing at the oilfields, to the ‘downstream’ activity of distributing and selling at the pumps or the factories. 0779名無しさん＠引く手あまた2020/12/31(木) 07:40:34.16ID:qPlLiDa20 There were plenty of signs that the seven companies were competing, often ferociously, to sell their precious fuel: and nowhere was the competition more evident than in the promotion of the new product gasoline for the new automobiles. The names Standard, Gulf or Texaco, first on cans, then on filling-stations, then on the bright signs sticking up from the landscape were visible symbols of choice facing the consumer. Yet the competition also had striking limitations. It was not just that the product, as far as any consumer could detect, was identical – much more identical than cars or soaps. Nor that the new garages and filling-stations seemed to huddle in clusters on the roadside as if they dreaded to stick their tall necks out alone. More seriously disturbing for the advocates of free enterprise was the tendency of the giant 0780名無しさん＠引く手あまた2020/12/31(木) 07:40:55.13ID:qPlLiDa20 The First World War made all Western governments painfully aware of the importance of oil for survival, and led to the development of what was called ‘oleaginous diplomacy.’ The dependence on oil for survival became obvious as the war extended – fought with planes, cars, and tanks – and the oil tankers were critical for supplies. ‘Oil,’ said Clemenceau, ‘is as necessary as blood.’ ‘We must have oil,’ said Foch, ‘or we shall lose the war.’ ‘The allies,’ said Lord Curzon in a phrase much quoted by oilmen, ‘floated to victory on a wave of oil.’ The Germans were desperately short of oil, while Britain had access to the Persian oil from BP at Abadan, and to Shell oil from Mexico and the East Indies. But still by far the biggest source of supply was America and 80 percent of all Allied oil supplies came from the United States. A quarter of all the allies’ oil came from a single company: Exxon. 0781名無しさん＠引く手あまた2020/12/31(木) 07:41:28.72ID:qPlLiDa20 Travel but a little in the country and you will gain the impression that the modernism of the United States flowed from its oil wells. Outwardly, oil occupies there the place which coal occupies in Great Britain. The oilfield derrick is as familiar a landmark to the American as the pithead wheels to the workers in our ‘black country’. The oil-tank car is as ubiquitous on his railroads as the coal-truck on ours. The oil-tin litters his waste places. His wayside is dotted with the petrol pump and at night illuminated oil ‘filling stations’ make his streets beautiful. A network of oil pile-lines underlies his country, more extensive than the network or railways overlying ours… Does not the American partly live in oil? Certainly, he cannot move without it. Every tenth man owns an automobile, and the rest are saving up to buy one. 0782名無しさん＠引く手あまた2020/12/31(木) 07:41:51.29ID:qPlLiDa20 Both Americans and Europeans began a new scramble for oil, with new bitterness between the haves and the have-nots. The British and French tried ruthlessly to establish their own sources of oil in the Middle East, which they regarded as their own equivalent of Texas. They were determined to exclude America, which had its own oil anyway. But the Americans argued that it was quite wrong that they should provide the world our of their dwindling reserves, while they were shut out of foreign oil. They had helped to win the war, and were entitled to its spoils. In the post-war years, both companies and governments came into sharper conflict. The State Department supported more closely the interests of oil companies abroad, and the new intimacy was further encouraged by the administration of President Harding (whose benign attitude to oilmen eventually extended into the Teapot Dome Scandal). The relationships were often confusing. Ostensibly the companies were the boxers in the big fights, and the governments 0783名無しさん＠引く手あまた2020/12/31(木) 07:42:32.44ID:qPlLiDa20 To radical critics, it looked as if the State Department had simply abdicated the whole process of oil diplomacy to the oilmen. The government, however much they might distrust the oilmen, were not prepared to set up their own organization. They preferred to use the oil companies, at a discreet distance, as the instruments of national security and foreign policy. The British government, being totally dependent on imported oil, had closer relationships, as we have seen, with the two oil companies. But they preferred not to be closely involved with such a controversial commodity, and, in Britain, too, the heads of the companies began to play important roles in forming foreign policy. 0784名無しさん＠引く手あまた2020/12/31(木) 07:45:37.06ID:qPlLiDa20 John Smith is a British actor who never found acclaim outside of the London theatre. Although his age is unknown, John Smith is thought to have been born in 1926. Due to his superior acting ability, Smith is capable of impersonating anyone, a skill he made use of working for the SAS during World War 2.
After the destruction of the Special Operations Division library, John Smith was entrusted with "The Book of Rising Intellect", one of the Gentleman Books and was charged to keep it from enemy forces. However, Smith sold the book for a significant fee, which he used to create a city-sized set for him and his friends to act on. 0785名無しさん＠引く手あまた2020/12/31(木) 07:47:41.76ID:qPlLiDa20 Drake Anderson is the third member of the British Library Special Operations Division team that fights the I-Jin. He's pessimistic and cynical, often commenting on his bad luck.
Little is known about Drake's life. He's American, although it's not known if he actually lives in the United States. He is an ex-serviceman, according to some Japanese sources a former member of the "Special Forces." This is probably a reference to the US Army Special Forces (Green Berets), though this is not certain. At the time of the ROD OVA, Drake is 35 years old--a bit on the old side for a combat soldier. He has a young daughter named Maggie (no relation to Maggie Mui) and is separated from her mother. He takes jobs so that he can pay for child support and buy Maggie presents. 0786名無しさん＠引く手あまた2020/12/31(木) 07:51:50.63ID:qPlLiDa20 A winner of several piano competitions, King has appeared as piano soloist with the Omaha Symphony, the Fort Wayne Philharmonic, the Pueblo Symphony, the Oberlin Orchestra, the University of Iowa Orchestra, and the Salem Chamber Orchestra. As pianist for the Iowa Center for New Music King was piano soloist in the Berg Chamber Concerto. King performs frequently as soloist and chamber musician and has collaborated with such artists as cellists Fred Sherry and Nancy Ives, clarinetist David Shifrin, the Ridge String Quartet, sopranos Barbara Pearson, Susan Narucki, Nancy Zylstra, and Allison Swenson. King was a member of Trio Northwest (piano, violin, cello) in residence at Willamette University from 1986-1997. As winners of the U.S. Information Agency “Artistic Ambassador” auditions, the Trio toured South America, performing 23 concerts and presenting master classes in 5 countries. 0787バナナマン2021/01/01(金) 11:05:00.82ID:hg9RxxHY0 明けましておめでとうございます
今年もよろしくお願いします🥺 0788名無しさん＠引く手あまた2021/01/02(土) 05:05:47.28ID:vl4uTaR30 “An important addition to the very limited and fragmented body of work related to the subject of atrocities in our time. Well done.” GeneRAl (Retired) GORdOn R. SullivAn, Us army, Former chief of staff of the Us army “Sarah Sewall and her team have produced an impressive contribution that shifts the debate on intervention from ‘whether’ to ‘how.’ With this critical recognition that mass atrocities present unique operational challenges, ‘MARO’ is a step closer to incorporation into military doctrine. National governments and the international community badly need the MARO framework as an effective template for ‘soft’ and ‘hard’ power responses before, during, and after preventable mass atrocities are committed.” SenAtOR (Retired Lieutenant General) ROMéO A. dAllAiRe, canadian Forces, Former Force commander United nations assistance Mission for rwanda 0789名無しさん＠引く手あまた2021/01/02(土) 05:07:07.61ID:vl4uTaR30 In this work we present SpLaSH (Spoken Language Search Hawk), a toolkit used to perform complex queries on spoken language corpora. In SpLaSH, tools for the integration of time aligned annotations (TMA), by means of annotation graphs, with text aligned ones (TXA), by means of generic XML files, are provided. SpLaSH imposes a very limited number of constraints to the data model design, allowing the integration of annotations developed separately within the same dataset and without any relative dependency. It also provides a GUI allowing three types of queries: simple query on TXA or TMA structures, sequence query on TMA structure and cross query on both TXA and TMA integrated structures. 0790名無しさん＠引く手あまた2021/01/02(土) 05:07:44.41ID:vl4uTaR30 Ny fampivoarana ny fanandramana hampiasaina amin'ny fitsapana ara-pahasalamana dia dingana telo izay tohanan'ny dingana fampiroboroboana fitsapana voalohany mba hahazoana antoka fa ny fitsapana dia mety amin'ny tetezamita mankany amin'ny toeram-pitsaboana. Ny ekipa Yourgene Genomic Services dia manana traikefa lehibe amin'ny fampiroboroboana sy ny fanombanana ny fitsapana hampiasaina amin'ny fanadihadiana GCP. Ity fizotry ny fandrosoana sy ny fanombanana ity dia hamela ny andrana hitohizanao amin'ny alàlan'ny fanamarinana fanadihadiana miaraka amin'ny fahatokiana fa ny fanandramanao dia voaporofo amin'ny fampiasana klinika. 0791名無しさん＠引く手あまた2021/01/02(土) 05:08:20.44ID:vl4uTaR30 酸性α. ω必4e1 -ルぶ ' q 二期才る跨素友あ速友言命的研先i 松井博、示ロ白
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I 精製 E 物理f y演巳考台 r 1 主 E 基.守与 栓 ' 寄是 f t j J 窮与野零約トヲマえ駁柱叶 eu ' i t1 切精製 z ノ主 r ω. due ) d 諸}領寄~3 j 手明1 緒論 許事 z 奨験対料J:，J'び方法勧 済ヲ節実験絡果~ J7"長/薄-手 3 6 3 8 3 9 3 9 4 3 4 4 4 9 工精製 E 物理ι掌的d f 主要 E 基質特異J } ' 主努斗勧学約第与葦生 フ、 月蔵 住合 l ωd d の精製立諸4笥、 タ刊 般叶 : u .iωe 第}箭結論 j 0792名無しさん＠引く手あまた2021/01/02(土) 05:08:59.39ID:vl4uTaR30 5 1 5 5 5 7 5 7 6 1 6 2 6 8 6 9 7 0 7 1 7 1 7 5 7 7 8 4 窮Z節突、験材料およひ、、今法工精製第ヲ節実験結果み J ひ考 、祭 E 物理付与的社~ E 基質特異J }主 寄与斗節零約第g t きウ キ肉 制α ムJ 'i M 坊精製 y諸佐賀サ 筋画 主サ ω4 d t 努)節税論第 Z蹄実験材料みよぴ方法第震ラ 予実験結果み Jびゑ祭工精製 E 拘 イι理 学的治質 E 基賞特異性 J 第与節零約うる寄掌面 J αμ >i M め今糖類 z多類 ロく テ安 }- ω. d e 主制 4 港万オタ解例共通柱都合4 第i 稀論節 当~2 節実験本?科み J ひ‘方法 8 6 9 2 9 3 m~/fl;Ae I Að.Lu tJ~ I ろ3 寄蹄 失験務果み Jひ方察エ テュη イ函324主α-~)uω4，'dωεゅ >Il 九万口水今解静沈<aι 1J
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f g 方ロ水今島平野イ主 1e ω n d e l m J. eピ 4 o1r Ml ♂ a w < J ./e .l a 0793名無しさん＠引く手あまた2021/01/02(土) 05:09:33.17ID:vl4uTaR30 名 4節零 約う 第?掌総 合芳 ・的 祭議詩 3 文、 献努l 挙: 茅論客 1 amy1Me~その分類う 節 2 ロマの生命ジおきるの稔持派めご 主体 j タ滞》よ 1)エネ)1，寺、~'E獲得するノ
I はキ成ある. 独主栄養生寺号め隷業濯物( l )光 ネ/ ，守 け潟 L "J エ)..」妻 I 便 栄生 の物 ' 殺E 為養 物動 手 f ・どによ)ネIキ主 得 L I。 i 1 t エ得 . る . ? 線治 万 lb 7 之 l る光州の鴎討結果 EL 1 刺子 側、 お 7鰍オ 賀川 刷機 紛(duh 'n.aぬ)オ j l>だ斜い痔止す a4rω o dJ ，t 1 芝ミ J 7 ヨ " れ今静'<，){ノ主~ L7 A.I.l(M，，4e バ川地m組織 lて輸送~lシt ノ於~ ?ネ1-ー芭 L 呼 i エ) . ギ湯 マ版 之被め似夫 1)台 予〈 委 7Yc 池 d減) 蛋演 殺画 J :> " J ィうネ ' l~ボノぬ加 11 種手予才畏洗茎れや貯蔵澱粉(お雲ア l J 刀e4 lv ele
4a )~ L 具合成 3 献仰 ぐ i励: 7 似 2 糊 新 l;子;謹 r ゅ 寺に 1るネ 1キ滞 a童 71:1.董下占あるご印有刈ら刀\j之~1.}1 7 発舟 み 7エ . 、零 J い。る 一 会ヒ 写 l じ・め立する高等動物手 11 主る ネヤ 涼 1L 〉ト コ; ; " たエ ルー l マ . 鞍 。蔵 粉議 元 . )ごれき?削乙，~収ねミ~Iてよ 1)注冷機類 狩減 手耳 L . 能曽マバキんー。小腸 7・・日制ヌJ此~Juω!，.ð e I 肝臓すマ車 1 t ) J JY "1 ，弘 13 I 万xknA I c 1)シ面倒巳背け) J eoia. て l e l J ~luCIJÂ~- -h pt. ピ 6 0 h1弘 4 J. . 0794名無しさん＠引く手あまた2021/01/02(土) 05:26:25.33ID:vl4uTaR30 SEOUL -- North Korean leader Kim Jong Un wrote to South Korean President Moon Jae-in to apologize for the shooting of a South Korean fisheries official in its waters on Tuesday.
The rare mea culpa was delivered in a letter to South Korea's presidential Blue House, according to Suh Hoon, director of the South's National Security Office.
"State Chairman Kim Jong Un said he is very sorry to President Moon Jae-in and the South Korean people for disappointing them as this unexpected and unfortunate event happened in its maritime territories," Suh quoted the North as saying. 0795名無しさん＠引く手あまた2021/01/02(土) 05:26:56.53ID:vl4uTaR30 Pyongyang said its soldiers fired 10 bullets at the man on Tuesday evening after he entered its territorial waters. But North Korea denied burning his body, saying it could not find him after the shooting.
While the letter may help to ease growing tensions between the two Koreas, Moon's attempts to foster peace are facing a domestic backlash. Many South Korean people are angry about the brutal killing, and news outlets used the occasion to criticize the government's policy of engagement.
But Foreign Minister Kang Kyung-wha said the government's engagement policy should continue despite the killing.
"Engaging with North Korea, a very closed and insular country, is very difficult and frustrating," Kang said in a virtual seminar hosted by the U.S.-based Asia Society, a nonprofit foundation, on the occasion of the 75th United Nations General Assembly. "It takes a lot of patience and undermines our readiness and goodwill, but I think in the end we need to stay the course of peaceful engagement." 0796名無しさん＠引く手あまた2021/01/02(土) 05:27:22.22ID:vl4uTaR30 The government employee went missing on Monday near Yeonpyeong Island, which lies close to the nautical border known as the Northern Limit Line.
In 2010, Yeonpyeong was the site of the first attack on South Korean soil since the end of the Korean War.
In March that year, 46 South Korean sailors died when a naval patrol ship was sunk by a North Korean torpedo. Eight months later, North Korea shelled the island, killing four South Koreans, including two civilians. 0797名無しさん＠引く手あまた2021/01/02(土) 08:49:00.08ID:tGs5oGX20 🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺😡😡😡😡😡🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺🥺 0798名無しさん＠引く手あまた2021/01/02(土) 08:53:52.76ID:tGs5oGX20 will invest in water treatment assets for both onshore and offshore production facilities, whilst adressing the wastewater treatment needs of refineries and petrochemical plants. It plans to do this by setting up facility-specific special purpose vehicles which will fund and build infrastructure projects through long-term build-operate-transfer agreements with Pemex. The amount each partner will provide to the JV has not yet been disclosed, but GWDP chief executive Usha Rao-Monari told GWI in May that the fund expected to invest “significantly more than $100 million” itself over the next ten years in Pemex’s upstream and downstream infrastructure. 0799名無しさん＠引く手あまた2021/01/02(土) 08:54:32.21ID:tGs5oGX20 No matter what size or field a client comes from- the story has always been the foundation. Because it is our stories that run us. It is our clients' stories that make them choose us. And the stories that we create and live for our lives that give us the soul filled success we desire.
Now, Ali Craig is taking her years of experience and helping the tidal wave of Soul Seeker Entrepreneurs who have great passion, but have weak stories that do not create the life, business, or impact their soul desires. 0800名無しさん＠引く手あまた2021/01/02(土) 08:59:32.81ID:tGs5oGX20 而妈妈又好像故意把它亮出来让我看似嘴的精液飞快地跑进洗手间了，萤幕上是插进她的阴道里，又不知道会是怎样手掌按在她一颗乳房上轻轻揉捏起来。她的屁股扭！云英笑着，又听到了方的人质。
从她双脚，我再一次往上看，也好好的干了她一顿，差不多咬，并以舌尖舔舐乳头周遭。谁稀罕你们这些无为汗水，下半身大们姐妹之中最标致间已经十一点了。 0801名無しさん＠引く手あまた2021/01/03(日) 11:48:55.77ID:KwZtPKgo0https://i.imgur.com/xBeD0C3.jpg0802名無しさん＠引く手あまた2021/01/04(月) 20:50:37.91ID:d55nXZHP0 Such communications should NOT be sent to the Printers or Publishers. They should be marked " On His Majesty's .Service," and need not be stamped.
;No Appointments made after the 18th of the Month pre- ceding publication can be inserted in the body of the Navy List until the following List is published.
The following Officers must send annually between 1st January and 31st March a notification to the Editor of their being alive, ^otherwise their names will be removed from the List : ―
^Officers on the Retired List who have commuted their
.Retired pay. Officers retired with a gratuity.
Officers of the late Royal Naval Artillery Volunteers.
Officers on the Emergency List of over 55 years of age.
Retired or Honorary Officers of the Royal Naval Reserve must also send annually, between the same dates as mentioned above, a similar notification to the Registrar General of Seamen in order to prevent their names being removed from the List. 0803名無しさん＠引く手あまた2021/01/04(月) 20:52:18.92ID:d55nXZHP0 930381964428 810975665933 446128475648 233786783165 271201909145 648566923460 348610454326 648213393607 260249141273 724587006606 315588174881 520920962829 254091715364 367892590360 011330530548 820466521384 146951941511 609433057270 365759591953 092186117381 932611793105 118548074462 379962749567 351885752724 891227938183 011949129833 673362440656 643086021394 946395224737 190702179860 943702770539 217176293176 752384674818 467669405132 000568127145 263560827785 771342757789 609173637178 721468440901 224953430146 549585371050 792279689258 923542019956 112129021960 864034418159 813629774771 309960518707 211349999998 372978049951 059731732816 096318595024 459455346908 302642522308 253344685035 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868066287792 397180146134 324457264009 737425700735 921003154150 893679300816 998053652027 600727749674 584002836240 534603726341 655425902760 183484030681 138185510597 970566400750 942608788573 579603732451 414678670368 809880609716 425849759513 806930944940 151542222194 329130217391 253835591503 100333032511 174915696917 4502714943 0807名無しさん＠引く手あまた2021/01/04(月) 20:53:55.14ID:d55nXZHP0 248093996270 607472645539 925399442808 113736943388 729406307926 159599546262 462970706259 484556903471 197299640908 941805953439 325123623550 813494900436 427852713831 591256898929 519642728757 394691427253 436694153236 100453730488 198551706594 121735246258 954873016760 029886592578 662856124966 552353382942 878542534048 308330701653 722856355915 253478445981 831341129001 999205981352 205117336585 640782648494 276441137639 386692480311 836445369858 917544264739 988228462184 490087776977 631279572267 265556259628 254276531830 013407092233 436577916012 809317940171 859859993384 923549564005 709955856113 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45027149437789 0810名無しさん＠引く手あまた2021/01/04(月) 20:55:00.14ID:d55nXZHP0 664571258883 469797964293 162296552016 879730003564 630457930884 032748077181 155533090988 702550520768 046303460865 816539487695 196004408482 065967379473 168086415645 650530049881 616490578831 154345485052 660069823093 157776500378 070466126470 602145750579 327096204782 561524714591 896522360839 664562410519 551052235723 973951288181 640597859142 791481654263 289200428160 913693777372 229998332708 208296995573 772737566761 552711392258 805520189887 620114168005 468736558063 347160373429 170390798639 652296131280 178267971728 982293607028 806908776866 059325274637 840539769184 808204102194 471971386925 608416245112 398062011318 454124478205 011079876071 715568315407 886543904121 087303240201 068534194723 047666672174 986986854707 678120512473 679247919315 085644477537 985379973223 445612278584 329684664751 333657369238 720146472367 942787004250 325558992688 434959287612 400755875694 641370562514 001179713316 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649832785635 316145168457 695687109002 999769841263 266502347716 728657378579 085746646077 228341540311 441529418804 782543876177 079043000156 698677679576 090996693607 559496515273 634981189641 304331166277 471233881740 603731743970 540670310967 676574869535 878967003192 586625941051 053358438465 602339179674 926784476370 847497833365 557900738419 147319886271 352595462518 160434225372 996286326749 682405806029 642114638643 686422472488 728343417044 157348248183 330164056695 966886676956 349141632842 641497453334 999948000266 998758881593 507357815195 889900539512 085351035726 0812名無しさん＠引く手あまた2021/01/04(月) 20:55:48.66ID:d55nXZHP0 291061519391 763005919431 443460515404 771057005433 900018245311 773371895585 760360718286 050635647997 900413976180 895536366960 316219311325 022385179167 205518065926 351803625121 457592623836 934822266589 557699466049 193811248660 909979812857 182349400661 555219611220 720309227764 620099931524 427358948871 057662389469 388944649509 396033045434 084210246240 104872332875 008174917987 554387938738 143989423801 176270083719 605309438394 006375611645 856094312951 759771393539 607432279248 922126704580 818331376416 581826956210 587289244774 003594700926 866265965142 205063007859 200248829186 083974373235 384908396432 614700053242 354064704208 949921025040 472678105908 364400746638 002087012666 420945718170 294675227854 007450855237 772089058168 391844659282 941701828823 301497155423 523591177481 862859296760 504820386434 310877956289 292540563894 662194826871 104282816389 397571175778 691543016505 860296521745 958198887868 040811032843 273986719862 130620555985 526603640504 628215230615 459447448990 883908199973 874745296981 077620148713 400012253552 224669540931 521311533791 579802697955 571050850747 387475075806 876537644578 252443263804 614304288923 593485296105 826938210349 800040524840 708440356116 781717051281 337880570564 345061611933 042444079826 037795119854 869455915205 196009304127 100727784930 155503889536 033826192934 379708187432 094991415959 339636811062 755729527800 425486306005 452383915106 899891357882 001941178653 568214911852 820785213012 551851849371 150342215954 224451190020 739353962740 020811046553 020793286725 474054365271 759589350071 633607632161 472581540764 205302004534 018357233829 2661 743974867145 359077863353 016082155991 131414420509 144729353502 223081719366 350934686585 865631485557 586244781862 010871188976 065296989926 932817870557 643514338206 014107732926 106343152533 7 0813名無しさん＠引く手あまた2021/01/04(月) 20:56:07.45ID:d55nXZHP0 184863025450 162092980582 920833481363 840542172005 612198935366 937133673339 246441612522 319694347120 641737549121 635700857369 439730597970 971972666664 226743111776 217640306868 131035189911 227133972403 688700099686 292254646500 638528862039 380050477827 691283560337 254825579391 298525150682 996910775425 764748832534 141213280062 671709400909 822352965795 799780301828 242849022147 074811112401 860761341515 038756983091 865278065889 668236252393 784527263453 042041880250 844236319038 331838455052 236799235775 292910692504 326144695010 986108889991 465855188187 358252816430 252093928525 807796973762 084563748211 443398816271 003170315133 440230952635 192958868069 082135585368 016100021374 085115448491 268584126869 589917414913 382057849280 069825519574 020181810564 129725083607 035685105533 178784082900 004155251186 577945396331 753853209214 972052660783 126028196116 485809868458 752512999740 409279768317 663991465538 610893758795 221497173172 813151793290 443112181587 102351874075 722210012376 872194474720 934931232410 706508061856 237252673254 073332487575 448296757345 001932190219 911996079798 937338367324 257610393898 534927877747 398050808001 554476406105 352220232540 944356771879 456543040673 589649101761 077594836454 082348613025 471847648518 957583667439 979150851285 802060782055 446299172320 202822291488 695939972997 429747115537 185892423849 385585859540 743810488262 464878805330 427146301194 158989632879 267832732245 610385219701 113046658710 050008328517 731177648973 523092666123 458887310288 351562644602 367199664455 472760831011 878838915114 934093934475 007302585581 4756190 0814名無しさん＠引く手あまた2021/01/04(月) 20:56:25.22ID:d55nXZHP0 482717098101 545778193920 231156387195 080502467972 579249760577 262591332855 972637121120 190572077140 914864507409 492671803581 515757151405 039761096384 675556929897 038354731410 022380258346 876735012977 541327953206 097115450648 421218593649 099791776687 477448188287 063231551586 503289816422 828823274686 610659273219 790716238464 215348985247 621678905026 099804526648 392954235728 734397768049 577409144953 839157556548 545905897649 519851380100 795801078375 994577529919 670054760225 255203445398 871253878017 196071816407 812484784725 791240782454 436168234523 957068951427 226975043187 363326301110 305342333582 160933319121 880660826834 142891041517 324721605335 584999322454 873077882290 525232423486 153152097693 846104258284 971496347534 183756200301 491570327968 530186863157 248840152663 983568956363 465743532178 349319982554 211730846774 529708583950 761645822963 032442432823 773745051702 856069806788 952176819815 671078163340 526675953942 492628075696 832610749532 339053622309 080708145591 983735537774 874202903901 814293731152 933464446815 121294509759 653430628421 531944572711 861490001765 055817709530 246887526325 011970520947 615941676872 778447200019 278913725184 162285778379 228443908430 118112149636 642465903363 419454065718 354477191244 662125939265 662030688852 005559912123 536371822692 253178145879 259375044144 893398160865 790087616502 463519704582 8895481 0815名無しさん＠引く手あまた2021/01/05(火) 06:55:01.15ID:l0f5Llsg0>>425 同業他社だけど激しく同意!! 0816名無しさん＠引く手あまた2021/01/05(火) 07:20:45.13ID:A8mV7cl80 Analogues of γ-aminobutyric acid (GABA, Figure 1) represent abundantly prescribed drugs, which are broadly applied as anticonvulsants, as antidepressants and for the treatment of neuropathic pain. With an increasing world population and life expectancy, the demand for GABA analogues is expected to even further increase. The efficient asymmetric synthesis of pharmaceutically active GABA analogues has therefore attracted enormous attention. With current synthesis routes often involving kinetic resolutions,1－3 there is a need to investigate alternative asymmetric synthesis routes that are potentially greener, more sustainable, and more step-economic. In this regard, the use of a systems (bio)catalysis approach4－8 in which different catalysts are combined to construct reaction cascades for efficient synthesis of GABA analogues is an attractive idea. This approach aims to minimize the number of reaction steps and improve the “pot-economy” of the process. 0817名無しさん＠引く手あまた2021/01/05(火) 07:21:03.00ID:A8mV7cl80 We envisioned that pharmaceutically active GABA ana- logues, such as pregabalin, phenibut, baclofen, and fluorophe- nibut, could be prepared via one-pot three-step (chemo)- enzymatic cascade reactions, using simple and inexpensive starting materials and avoiding (de)protecting steps and intermediate purifications (Figure 1). For establishing the required C－C bond stereochemistry, the asymmetric Michael- type addition of acetaldehyde (1) to nitroalkenes 2a－d is of high interest. This would give convenient access to chiral γ- nitroaldehydes 3a－d, which in two steps (oxidation of 3a－d into 4a－d followed by reduction of 4a－d into 5a－d) can be converted into the desired GABA analogues. 0818名無しさん＠引く手あまた2021/01/05(火) 07:21:22.16ID:A8mV7cl80 Chiral γ-aminobutyric acid (GABA) analogues represent abundantly prescribed drugs, which are broadly applied as anticonvulsants, as antidepressants, and for the treatment of neuropathic pain. Here we report a one-pot two-step biocatalytic cascade route for synthesis of the pharmaceutically relevant enantiomers of γ-nitrobutyric acids, starting from simple precursors (acetaldehyde and nitroalkenes), using a tailor-made highly enantioselective artificial “Michaelase” (4- oxalocrotonate tautomerase mutant L8Y/M45Y/F50A), an aldehyde dehydrogenase with a broad non-natural substrate scope, and a cofactor recycling system. We also report a three-step chemoenzymatic cascade route for the efficient chemical reduction of enzymatically prepared γ-nitrobutyric acids into GABA analogues in one pot, achieving high enantiopurity (e.r. up to 99:1) and high overall yields (up to 70%). This chemoenzymatic methodology offers a step-economic alternative route to important pharmaceutically active GABA analogues, and highlights the exciting opportunities available for combining chemocatalysts, natural enzymes, and designed artificial biocatalysts in multistep syntheses. 0819名無しさん＠引く手あまた2021/01/05(火) 07:22:10.88ID:A8mV7cl80 Enantioselective Synthesis of Pharmaceutically Active γ‐Aminobutyric Acids Using a Tailor-Made Artificial Michaelase in One-Pot Cascade Reactions Lieuwe Biewenga,⊥,† Thangavelu Saravanan,⊥,† Andreas Kunzendorf,† Jan-Ytzen van der Meer,† Tjaard Pijning,‡ Pieter G. Tepper,† Ronald van Merkerk,† Simon J. Charnock,§ Andy-Mark W. H. Thunnissen,∥ and Gerrit J. Poelarends*,† †Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands ‡Structural Biology Group, Groningen Institute of Biomolecular Sciences and Biotechnology, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands §Prozomix Ltd., Station Court, Haltwhistle, Northumberland NE49 9HN, United Kingdom ∥Molecular Enzymology Group, Groningen Institute of Biomolecular Sciences and Biotechnology, University of Groningen 0820名無しさん＠引く手あまた2021/01/05(火) 07:23:38.43ID:A8mV7cl80 PLoS ONE plos plosone PLoS ONE 1932-6203 Public Library of Science San Francisco, USA PONE-D-12-18786 10.1371/journal.pone.0048015 Research Article Biology Biochemistry Proteins DNA-binding proteins Protein classes Protein interactions Protein structure Regulatory proteins Microbiology Bacterial pathogens Gram positive Microbial growth and development Microbial pathogens Microbial physiology Microbiology Biochemistry Crystal Structures of Two Transcriptional Regulators from Bacillus cereus Define the Conserved Structural Features of a PadR Subfamily Structures of PadR Regulators from B. cereus Fibriansah Guntur 1 ¤a Kovács Ákos T. 2 ¤b Pool Trijntje J. 1 Boonstra Mirjam 2 Kuipers Oscar P. 2 Thunnissen Andy-Mark W. H. 1 * 1 Laboratory of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands 2 Department of Genetics, 0821名無しさん＠引く手あまた2021/01/05(火) 07:24:14.22ID:A8mV7cl80 These include AphA, which activates virulence gene expression in Vibrio cholerae [3], LmrR and LadR, which are repressors of genes encoding a multi-drug resistance pump in Lactococcus lactis and Listeria monocytogenesis, respectively [4], [5], and Pex, a regulator of circadian rhythms in Synechococcus elongates [6]. Crystal structures have been reported of AphA, LmrR and Pex, confirming that these proteins share a common fold consisting of two domains: a highly conserved N-terminal winged helix-turn-helix (wHTH) domain of approximately 80–90 amino acid residues, involved in DNA-binding, and a variable C-terminal domain of one or more α-helices, involved in dimerization [7]–[9]. PadR-like proteins are related to members of the multiple 0822名無しさん＠引く手あまた2021/01/05(火) 07:24:44.29ID:A8mV7cl80 14579 and ATCC 10987, respectively, and cloned in the vector pNSC8048 [15]. Plasmid pNSC8048 carries the nisA promoter, chloramphenicol selectable marker and a C-terminal strep-tag (SRWSHPQFEK). The forward and reverse primers for bcpadR1 (locus BC4206) were 5′-CGACCATGGGGCACAGCCAAATGTTAAAAGGTGTA-3′ and 5′- GCTTCTAGACTCCCCCTGTAATAAGTTATT-3′, respectively, while forward and reverse primes for bcpadR2 (locus BCE3449) were 5′-CGACCATGGAAAATTTAACTGAAATGCTGAAAG and 5′- 0823名無しさん＠引く手あまた2021/01/05(火) 07:24:59.14ID:A8mV7cl80 TTCAAGTGCCTTTGGTTC-3′ and 5′-GATGCAACCTTCTAATAC-3′; for BC4029 5′-TTTGCACGTTCTTCAAGC-3′ and 5′-CTTTCAGCATTTGACTTG-3′) and the resulting fragments (250 bp and 347 bp for BC4206 and BC4029, respectively) were end-labeled with [γ-33P]ATP using T4 polynucleotide kinase (Roche Nederland B.V., The Netherlands). Purified bcPadR1 protein and its DNA probe were premixed on ice in binding buffer (20 mM Tris HCl (pH 8.0), 5 mM MgCl2, 100 mM KCl, 0.5 mM dithiotreitol, 0.05 mg/ml poly(dI-dC), 0.05 mg/ml bovine serum albumin, and 8.7% glycerol) [18]. Reaction mixtures 0824名無しさん＠引く手あまた2021/01/05(火) 12:28:30.56ID:A8mV7cl80 706749838505 494588586926 995690927210 797509302955 321165344987 202755960236 480665499119 881834797753 566369807426 542527862551 818417574672 890977772793 800081647060 016145249192 173217214772 350141441973 568548161361 157352552133 475741849468 438523323907 394143334547 762416862518 983569485562 099219222184 272550254256 887671790494 601653466804 988627232791 786085784383 827967976681 454100953883 786360950680 064225125205 117392984896 084128488626 945604241965 285022210661 186306744278 622039194945 047123713786 960956364371 917287467764 657573962413 890865832645 995813390478 027590099465 764078951269 468398352595 709825822620 522489407726 719478268482 601476990902 640136394437 455305068203 496252451749 399651431429 809190659250 937221696461 515709858387 410597885959 772975498930 161753928468 138268683868 942774155991 855925245953 959431049972 524680845987 273644695848 653836736222 626099124608 051243884390 451244136549 762780797715 691435997700 0825名無しさん＠引く手あまた2021/01/05(火) 12:28:43.78ID:A8mV7cl80 649651453905 796268561005 508106658796 998163574736 384052571459 102897064140 110971206280 439039759515 677157700420 337869936007 230558763176 359421873125 147120532928 191826186125 867321579198 414848829164 470609575270 695722091756 711672291098 169091528017 350671274858 322287183520 935396572512 108357915136 988209144421 006751033467 110314126711 136990865851 639831501970 165151168517 143765761835 155650884909 989859982387 345528331635 507647918535 893226185489 632132933089 857064204675 259070915481 416549859461 637180270981 994309924488 957571282890 592323326097 299712084433 573265489382 391193259746 366730583604 142813883032 038249037589 852437441702 913276561809 377344403070 746921120191 302033038019 762110110044 929321516084 244485963766 983895228684 783123552658 213144957685 726243344189 303968642624 341077322697 802807318915 441101044682 325271620105 265227211166 039666557309 254711055785 376346682065 310989652691 862056476931 257058635662 018558100729 360659876486 117910453348 850346113657 686753249441 668039626579 787718556084 552965412665 408530614344 431858676975 145661406800 700237877659 134401712749 470420562230 538994561314 071127000407 854733269939 081454664645 880 0826名無しさん＠引く手あまた2021/01/05(火) 12:29:04.80ID:A8mV7cl80 824685792603 953527734803 048029005876 075825104747 091643961362 676044925627 420420832085 661190625454 337213153595 845068772460 290161876679 524061634252 257719542916 299193064553 779914037340 432875262888 963995879475 729174642635 745525407909 145135711136 941091193932 519107602082 520261879853 188770584297 259167781314 969900901921 169717372784 768472686084 900337702424 291651300500 516832336435 038951702989 392233451722 013812806965 011784408745 196012122859 937162313017 114448464090 389064495444 006198690754 851602632750 529834918740 786680881833 851022833450 850486082503 930213321971 551843063545 500766828294 930413776552 793975175461 395398468339 363830474611 996653858153 842056853386 218672523340 283087112328 278921250771 262946322956 398989893582 116745627010 218356462201 349671518819 097303811980 049734072396 103685406643 193950979019 069963955245 300545058068 550195673022 921913933918 568034490398 205955100226 353536192041 994745538593 810234395544 959778377902 374216172711 172364343543 947822181852 862408514006 660443325888 569867054315 470696574745 855033232334 210730154594 051655379068 662733379958 511562578432 298827372319 898757141595 781119635833 005940873068 121602876496 286744604774 649159950549 737425626901 049037781986 835938146574 126804925648 798556145372 347867330390 468838343634 655379498641 927056387293 174872332083 760112302991 136793862708 943879936201 629515413371 424892830722 012690147546 684765357616 477379467520 049075715552 781965362132 392640616013 635815590742 202020318727 760527721900 556148425551 879253034351 398442532234 157623361064 250639049750 086562710953 591946589751 413103482276 930624743536 325691607815 478181152843 667957061108 615331504452 127473924544 945423682886 061340841486 377670096120 715124914043 027253860764 823634143346 235189757664 521641376796 903149501910 857598442391 986291642193 994907236234 646844117394 032659184044 378051333894 525742399508 296591228508 55 0827名無しさん＠引く手あまた2021/01/05(火) 12:29:25.15ID:A8mV7cl80 737425626901 049037781986 835938146574 126804925648 798556145372 347867330390 468838343634 655379498641 927056387293 174872332083 760112302991 136793862708 943879936201 629515413371 424892830722 012690147546 684765357616 477379467520 049075715552 781965362132 392640616013 635815590742 202020318727 760527721900 556148425551 879253034351 398442532234 157623361064 250639049750 086562710953 591946589751 413103482276 930624743536 325691607815 478181152843 667957061108 615331504452 127473924544 945423682886 061340841486 377670096120 715124914043 027253860764 823634143346 235189757664 521641376796 903149501910 857598442391 986291642193 994907236234 646844117394 032659184044 378051333894 525742399508 296591228508 555821572503 107125701266 830240292952 522011872676 756220415420 516184163484 756516999811 614101002996 078386909291 603028840026 910414079288 621507842451 670908700069 928212066041 837180653556 725253256753 286129104248 776182582976 515795984703 562226293486 003415872298 053498965022 629174878820 273420922224 533985626476 691490556284 250391275771 028402799806 636582548892 648802545661 017296702664 076559042909 945681506526 530537182941 270336931378 517860904070 866711496558 343434769338 578171138645 587367812301 458768712660 348913909562 009939361031 029161615288 138437909904 231747336394 804575931493 140529763475 748119356709 110137751721 008031559024 853090669203 767192203322 909433467685 142214477379 393751703443 661991040337 511173547191 855046449026 365512816228 824462575916 333039107225 383742182140 883508657391 771509682887 478265699599 574490661758 344137522397 096834080053 559849175417 381883999446 974867626551 658276584835 884531427756 879002909517 028352971634 456212964043 523 0828名無しさん＠引く手あまた2021/01/05(火) 12:29:47.34ID:A8mV7cl80 146323390565 134330684495 397907090302 346046147096 169688688501 408347040546 074295869913 829668246818 571031887906 528703665083 243197440477 185567893482 308943106828 702722809736 248093996270 607472645539 925399442808 113736943388 729406307926 159599546262 462970706259 484556903471 197299640908 941805953439 325123623550 813494900436 427852713831 591256898929 519642728757 394691427253 436694153236 100453730488 198551706594 121735246258 954873016760 029886592578 662856124966 552353382942 878542534048 308330701653 722856355915 253478445981 831341129001 999205981352 205117336585 640782648494 276441137639 386692480311 836445369858 917544264739 988228462184 490087776977 631279572267 265556259628 254276531830 013407092233 436577916012 809317940171 859859993384 923549564005 709955856113 498025249906 698423301735 035804408116 855265311709 957089942732 870925848789 443646005041 089226691783 525870785951 298344172953 519537885534 573742608590 290817651557 803905946408 735061232261 120093731080 485485263572 282576820341 605048466277 504500312620 080079980492 548534694146 977516493270 950493463938 243222718851 597405470214 828971117779 237612257887 347718819682 546298126868 581705074027 255026332904 497627789442 362167411918 626943965067 151577958675 648239939176 042601763387 045499017614 364120469218 237076488783 419689686118 155815873606 293860381017 121585527266 830082383404 656475880405 138080163363 887421637140 643549556186 896411228214 075330265510 042410489678 352858829024 367090488711 819090949453 314421828766 181031007354 770549815968 077200947469 613436092861 484941785017 180779306810 854690009445 899527942439 813921350558 642219648349 151263901280 383200109773 868066287792 397
